Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.
Viczian, A. S., Zuber, M. E. Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis. J. Vis. Exp. (39), e1932, doi:10.3791/1932 (2010).