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Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

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JoVE Journal Biology

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

DOI:

10.3791/1932-v

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11:00 min

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May 16, 2010

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Andrea S. Viczian, Michael E. Zuber

¹Department of Ophthalmology,SUNY Upstate Medical University

Chapters

00:00Title
00:59Introduction
01:24Setting Up for the ACT Assay
03:07Preparing for Microinjection
05:18Microinjection
06:30Isolating Animal Caps from Injected Embryos
07:49Transplantation of Caps onto Flank
09:53Animal Cap Transplant Assay Representative Results
10:34Conclusion

Summary

Automatic Translation

Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

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Chapters

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