Embryo Transplantation

Embryo transplantation is a reproductive biotechnology in which an early embryo is removed from a donor and placed in the reproductive tract of a recipient, allowing gestation to occur in a different individual. The procedure typically involves fertilizing the donor’s oocytes, recovering embryos at an early developmental stage, and transferring them into a recipient whose reproductive cycle is synchronized with the embryo’s stage so implantation can occur. In biology, embryo transplantation supports the study of mammalian development and reproductive physiology, while applications in animal breeding can multiply valuable genetic traits, improve herd management, and aid the preservation of endangered species.

Embryo Transplantation - Related Videos

Research

JoVE Journal - Biology

Generating Chimeric Zebrafish Embryos by Transplantation

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Cited by 61 •

2009

A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

Facial Transplants in Xenopus laevis Embryos

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Cited by 10 •

2014

A technique for transplanting "Extreme Anterior Domain" facial tissue between Xenopus laevis embryos has been developed. Tissue can be moved from one gene expression background into another, allowing the study of local requirements for craniofacial development and for signaling interactions between facial regions.

Research

JoVE Journal - Biology
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Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

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Cited by 20 •

2010

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

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Cited by 4 •

2016

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

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Cited by 4 •

2010

We demonstrate a protocol to generate chimeric zebrafish embryos for live imaging cellular behavior during embryogenesis.

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