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审核端粒G -悬结构锥虫布氏</em
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Immunology and Infection
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JoVE Journal Immunology and Infection
Examination of the Telomere G-overhang Structure in Trypanosoma brucei
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审核端粒G -悬结构锥虫布氏</em

DOI:
10.3791/1959-v

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15:25 min

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January 26, 2011

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1Biological, Geo. & Env. Sciences,Cleveland State University

Chapters

  • 00:05Title
  • 01:12Introduction
  • 01:59Detection of T. brucei Telomere G-overhang Using Native In-Gel Hybridization
  • 07:18Determination of the Telomere Nucleotide by Adaptor Ligation
  • 09:48Ligation Mediated Primer Extension
  • 11:46Representative Results for the Examination of the Telomere G-overhang Structure
  • 15:00Conclusion

Summary

Automatic Translation

Automatic Translation

端粒是染色体的稳定至关重要，并介导的端粒酶的端粒维持端粒G -悬结构是必不可少的。最近，我们通过两种方法检测端粒G -悬结构<em>锥虫布氏</em>，这是原生 - 凝胶杂交和连接介导的引物延伸，这将是描述。

Tags

Telomere G-overhang StructureTrypanosoma BruceiTelomeraseTelomere MaintenanceSleeping SicknessNaganaSurface Antigen Gene ExpressionT. Brucei PathogenesisNative In-gel HybridizationLigation-mediated Primer ExtensionAdaptor Ligation MethodTelomere Terminal Nucleotide

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