Journal
/
Biology
/
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

DOI:
10.3791/1960-v

•

08:11 min

•

May 19, 2010

•

, ,
1Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine,School of Medicine, University of California, Davis

Chapters

  • 00:00Title
  • 01:03Introduction
  • 01:49Passaging Mouse Embryonic Stem Cells
  • 03:18Generation Of Oligodendrocyte Precursor Cells From Mouse ES Cells
  • 05:40Generation of Spiking Oligodendrocyte Precursor Cells
  • 06:47Representative Results
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

Tags

Embryonic Stem CellsOligodendrocyte PrecursorsMyelinating CellsCentral Nervous SystemRegenerative Cell TherapyDemyelinating DiseasesTransplantationTranscription Factor Olig2Proteoglycan NG2GFP-Olig2 Mouse Embryonic Stem Cell Reporter LineDifferentiationSmall MoleculesRetinoic AcidSonic Hedgehog Agonist PurmorphamineElectrophysiological PropertyNaV1.2 Subunit

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1960?article_type=v

Propagation of Human Embryonic Stem (ES) Cells
check_url/1960?article_type=v

Culture of Mouse Neural Stem Cell Precursors
check_url/1960?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/1960?article_type=v

Derivation of Human Embryonic Stem Cells by Immunosurgery
check_url/1960?article_type=v

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
check_url/1960?article_type=v

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
check_url/1960?article_type=v

Isolation and Derivation of Mouse Embryonic Germinal Cells
check_url/1960?article_type=v

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/1960?article_type=v

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
check_url/1960?article_type=v

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Read Article