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JoVE Journal
Biology

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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
 

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Article DOI: 10.3791/1960
May 19th, 2010

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Summary May 19th, 2010

We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

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