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Derivation of Mouse Trophoblast Stem Cells from Blastocysts
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Derivation of Mouse Trophoblast Stem Cells from Blastocysts

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

DOI:
10.3791/1964-v

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10:19 min

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June 08, 2010

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, ,
1Department of Biomedical Genetics, Center for Oral Biology, James P Wilmot Cancer Center,University of Rochester

Chapters

  • 00:00Title
  • 01:12Introduction
  • 01:41Derivation and Maintenance of Trophoblast Stem (TS) Cells
  • 07:40Freezing and Thawing TS cells
  • 08:46Differentiation of TS Cells into Trophoblast Giant Cells in vitro
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, we demonstrate the isolation of mouse blastocysts and the derivation of trophoblast stem cells from blastocysts. We also describe conditions for maintenance of the stem cell property as well as induction of differentiation in culture.

Tags

Mouse Trophoblast Stem CellsBlastocystsTrophectodermPlacenta DevelopmentTS CellsTanaka Et Al. 1998Trophoblast LineageTrophoblast Specific PropertyPlacentation EventsTrophoblast PolyploidizationGenome AmplificationRegulatory Mechanism Of Genome Replication And InstabilityChiu Et Al. 2008

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