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Biology

In situ Quantification of Pancreatic Beta-cell Mass in Mice

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Biology

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JoVE Journal Biology

In situ Quantification of Pancreatic Beta-cell Mass in Mice

In situ Quantification of Pancreatic Beta-cell Mass in Mice

DOI:

10.3791/1970-v

•

09:50 min

•

June 07, 2010

•

Abraham Kim, German Kilimnik, Manami Hara

¹Department of Medicine,University of Chicago

Chapters

00:00Title
00:57Introduction
01:23Preparation of Specimens
04:19Imaging and Quantifying the Beta-Cell Area in the Intact Pancreas
07:58Slice Image Analysis of Beta Cells in an Intact Adult Pancreas
09:27Conclusion

Summary

Automatic Translation

The following protocol outlines the process of pancreatic dissection for virtual slice imaging, and the subsequent quantification of all GFP-tagged beta-cells in the entire pancreas.

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