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Photoconversion van gezuiverde fluorescerende eiwitten en dual-probe optische markering in levende cellen
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Photoconversion van gezuiverde fluorescerende eiwitten en dual-probe optische markering in levende cellen

Article DOI: 10.3791/1995-v • 11:22 min • June 26th, 2010
June 26th, 2010 •
1, 1
1Department of Molecular Physiology and Biophysics, Vanderbilt University

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Dit protocol beschrijft een algemene aanpak voor photoconversion van fluorescerende eiwitten te voeren op een confocale laser scanning microscoop. We beschrijven de procedures voor de photoconversion van puried eiwit monsters, evenals voor dual-probe optische markeren in levende cellen met mOrange2 en Dronpa.

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Cellular Biology Morange Dronpa photoconversion fotoactivatie octanol druppel confocale imaging
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