This video demonstrates the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. Views of live cultures show neurite outgrowth and imaging of calcium levels using Fura-2.
Sicaeros, B., Campusano, J. M., O'Dowd, D. K. Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. J. Vis. Exp. (4), e200, doi:10.3791/200 (2007).