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(充电)酰化tRNA的使用微阵列技术水平的全基因组分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
DOI:

07:32 min

June 18, 2010

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Chapters

  • 00:00Title
  • 00:45Introductions
  • 01:06Isolation of Total Charged tRNA
  • 02:37Cy3/Cy5 Labeling of tRNA
  • 05:15Hybridization and Analysis of the Microarray
  • 06:33Representative Results of Isolation and Analysis of tRNA
  • 07:09Conclusion

Summary

Automatic Translation

我们描述了一种用于微阵列分析的方法来确定所有的tRNA相对酰化水平<em> S。 cerivisiae。</em

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