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Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
JoVE Journal
Biology
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JoVE Journal Biology
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
DOI:

08:56 min

August 01, 2010

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Chapters

  • 00:04Title
  • 00:56Introduction
  • 01:44Preparation of hESC Pellet
  • 03:51Renal Capsule Grafting
  • 06:23Teratoma Harvest, Fixation, and Sectioning
  • 07:21Representative Images of Teratoma Sections
  • 08:25Conclusion

Summary

Automatic Translation

Directed differentiation of hESCs into specific cells has generated much interest in regenerative medicine. We provide a concise, step-by-step protocol for determining the in vivo fate of selected hESCs that provides a valuable tool for characterizing tissue-specific reagents for cell-based therapy.

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