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Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
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Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels

DOI:
10.3791/2089-v

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10:19 min

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August 10, 2010

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, ,
1Department of Pharmacology,University of Pennsylvania

Chapters

  • 00:04Title
  • 00:49Introduction
  • 01:10Coverslip Preparation
  • 03:50Preparation of Hydrogels
  • 06:15Modeling
  • 08:35Representative Results
  • 09:50Conclustion

Summary

Automatic Translation

Automatic Translation

The effect of substrata stiffness on cellular function can be modeled in vitro using polyacrylamide hydrogels of varying compliances.

Tags

Matrix StiffnessCellular FunctionAcrylamide-based HydrogelsFibrosisCancerCardiovascular DiseaseCell BiologyECM-coated HydrogelsPolyacrylamideNaOH3-APTMSGlutaraldehydeAcrylamide (AC)Bis-acrylamide (Bis-AC)Ammonium PersulfateN-hydroxysuccinimide (NHS)RheologyAtomic Force Microscopy (AFM)

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