Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions

The overall goal of this procedure is to induce and visualize cyst wall formation in Toxoplasma Gandhi. In vitro. This is accomplished by first culturing macrophages and fibroblasts.

And then by infecting these host cells with the parasite stress responses can be induced in te gde eye by shocking fibroblasts with pH or activating the macrophages, which will both lead to cyst wall formation. Finally, fluorescent lectin is added to the cultures which binds to the cyst wall, such that cyst can be visualized with immunofluorescence microscopy. Hi, I am Crystal Tobin from the laboratory of Dr.Laura Noel in the Department of Medical Microbiology and Immunology at the University of Wisconsin Madison.