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Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering
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Bioengineering
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JoVE Journal Bioengineering
Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

DOI:
10.3791/2109-v

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11:32 min

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December 20, 2010

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, , , ,
1University of California, San Diego

Chapters

  • 00:05Title
  • 00:59Introduction
  • 01:34Pre-processing Tissue Preparation
  • 02:52Preparation of Solubilized Extracellular Matrix
  • 04:56Myocardial Injection
  • 09:57Representative Immunohistochemistry
  • 11:02Conclusion

Summary

Automatic Translation

Automatic Translation

Methods for preparing an injectable matrix gel from decellularized tissue and injecting it into rat myocardium in vivo are described.

Tags

Biologically Derived Injectable MaterialsExtracellular Matrix GelLyophilizationEnzymatic DigestionPhysiological PHECM PowderPepsin DigestionInjectable MatrixCharacterization AssaysNative ECM ComponentsProteinsPeptidesGlycosaminoglycansIntramural InjectionLeft Ventricular Free WallSmall Animal ModelBiotin-labelingHorse Radish Peroxidase-conjugated Neutravidin

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