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JoVE Journal Biology
Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
 

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties

Article doi: 10.3791/2131
October 13th, 2010 •
1, 1, 1, 1
1Department of Physiology and Biophysics, Weill Cornell Medical College

Summary
Automatic Translation
October 13th, 2010

We introduce a fast fluorescence-based assay that monitors the rate of fluorescence quenching as a measure of gramicidin channel activity. The gramicidin channels are used as molecular force transducers to monitor changes in lipid bilayer properties as sensed by bilayer spanning proteins.

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