Biology
A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.
Summary December 3rd, 2010
In this protocol we demonstrate the expression, solubilization, and purification of a recombinantly expressed membrane protein, MexB, as a soluble protein detergent complex. MexB is a multidrug resistance membrane transporter from the opportunistic bacterial pathogen Pseudomonas aeruginosa.