Stomatal Aperture Measurement
Stomatal Aperture Measurement - Related Videos
07:03
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
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Cited by 38 •
2010
We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
11:09
Relating Stomatal Conductance to Leaf Functional Traits
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Cited by 39 •
2015
Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
12:11
Measurement of Leaf Hydraulic Conductance and Stomatal Conductance and Their Responses to Irradiance and Dehydration Using the Evaporative Flux Method (EFM)
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Cited by 105 •
2012
We describe a relatively rapid (30 min) and realistic method for simultaneously measurement of leaf hydraulic conductance (Kleaf) and stomatal conductance (gs) for transpiring excised leaves. The method can be modified to measure the light and dehydration responses of Kleaf and gs.
05:03
Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana
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Cited by 1 •
2024
Here, we present a simple method for direct observation and automated measurement of stomatal responses to bacterial invasion in Arabidopsis thaliana. This method leverages a portable stomatal imaging device, together with an image analysis pipeline designed for leaf images captured by the device.
13:07
Convergent Polishing: A Simple, Rapid, Full Aperture Polishing Process of High Quality Optical Flats & Spheres
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Cited by 11 •
2014
A novel optical polishing process, called “Convergent Polishing”, which enables faster, lower cost polishing, is described. Unlike conventional polishing processes, Convergent Polishing allows a glass workpiece to be polished in a single iteration and with high surface quality to its final surface figure without requiring changes to polishing parameters.
FAQs
Guard-cell ion transport changes osmotic conditions inside the guard cells, altering their turgor and therefore pore dimensions. As turgor shifts, paired guard cells adjust the aperture, allowing measurements to connect visible width or area with underlying physiological regulation. This mechanistic link helps researchers interpret whether a treatment promotes opening or closure under defined conditions.
Pathogens, microbial signals, and plant immune responses can shift stomatal behavior, so measured aperture becomes an early readout of host-microbe interaction. A decrease may indicate closure-associated defense behavior, whereas increased or maintained opening may reflect a different response under the tested conditions. The measurement links visible pore changes with infection-related regulation.
The result depends on both the biological treatment and the measurement variable selected. Width records the opening across a pore, whereas area captures the overall two-dimensional extent of that opening in an image. Keeping conditions defined and applying the same measurement choice across samples makes comparisons of pathogen, microbial-signal, or immune-response effects more interpretable.
A basic workflow begins by exposing leaves to the defined condition of interest, imaging stomata with light or confocal microscopy, and using image analysis to quantify pore width or area. Researchers then compare those measurements across the tested conditions. This sequence converts visual changes in guard-cell pores into numerical data for evaluating treatment-associated stomatal responses.
Depending on the experimental setup, investigators may use light microscopy or confocal microscopy, followed by image analysis. The essential output is a measurable pore dimension, expressed as width or area, rather than a purely descriptive image. Consistent imaging and analysis across conditions support direct comparison of stomatal responses to tested factors.
In immunology and infection research, the method is especially useful for examining early plant defense responses. Because stomata can serve as microbial entry points while regulating gas exchange, researchers can assess whether pathogens or microbial signals are associated with altered aperture under defined conditions. These data help identify factors linked to plant susceptibility or resistance.