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JoVE Journal
Immunology and Infection

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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
 

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

Article DOI: 10.3791/2186
October 6th, 2010

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Summary October 6th, 2010

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

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