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JoVE Journal
Immunology and Infection

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Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
 

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

Article DOI: 10.3791/2195
December 18th, 2010

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Summary December 18th, 2010

The colony forming cell (CFC) assay is an in vitro assay in which hematopoietic progenitors form colonies in a semi-solid medium. A combination of colony morphology, cell morphology, and flow cytometry are used to assess the ability of the progenitors to proliferate and differentiate along the different hematopoietic lineages.

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