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JoVE Journal
Biology

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Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
 

Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination

Article DOI: 10.3791/222
July 4th, 2007

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Summary July 4th, 2007

Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.

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