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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

DOI:
10.3791/2220-v

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10:36 min

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December 21, 2010

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, ,
1Department of Biology,Portland State University

Chapters

  • 00:05Title
  • 00:57Introduction
  • 01:19Cell Growth and UV Irradiation
  • 03:14DNA Isolation
  • 05:502D Gel and Southern Analysis
  • 08:57Representative Results for Visualization of UV-induced Replication Intermediates in E. coli
  • 10:13Conclusion

Summary

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Automatic Translation

We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.

Tags

UV-induced Replication IntermediatesE. ColiTwo-dimensional Agarose-gel AnalysisInaccurate ReplicationDNA DamageCellular RearrangementsMutagenesisCancer DevelopmentGene ProductsReplication Encounters DNA LesionsReplicating PlasmidsUV-induced DNA DamageReplication ForksRecARecF PathwayNucleotide Excision Repair

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