We present a procedure by which two-dimensional agarose-gel analysis can be used to identify the structure of replication intermediates that occur following UV irradiation.
Jeiranian, H. A., Schalow, B. J., Courcelle, J. Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis. J. Vis. Exp. (46), e2220, doi:10.3791/2220 (2010).