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Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
 

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Article DOI: 10.3791/2244
September 17th, 2010

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Summary September 17th, 2010

This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.

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