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抗原特異的インビボでのキリングアッセイ
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
DOI:

09:49 min

November 09, 2010

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Chapters

  • 00:00Title
  • 01:13Habituating Animals
  • 01:29Eliciting Target-Specific Effector CTLs by Immunization (Day 0)
  • 03:03Program Operant Test Session
  • 03:16Harvesting Target Cells (Day 8)
  • 05:32Treatment and Labeling of Target Cells
  • 06:33Results: Operant Sensation Seeking Behavior
  • 07:39Adoptive Transfer and Subsequent Harvest of Labeled Cells
  • 08:06Conclusion
  • 08:20Flow Cytometry and Data Analysis
  • 08:36CFSE Profile Demonstrating Cell-Specific Killing in BALB/c Mice
  • 09:34Conclusion

Summary

Automatic Translation

多くの感染症は、強力なCTL応答を誘発するが、時折、応答する細胞の量は、病原体の制御に相関しない<sup> 1</sup>。 CTLの質の1つの尺度は具体的に死滅させる能力です。<sup> 2</sup>。標的細胞のCFSE標識は、このCTL応答の品質を調査するために使用することができます<em> in vivoで</em<sup> 3,4</sup>。

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