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January 11, 2011
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Summary
Automatically generated
The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.
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Cite this Article
Zhang, Y., Kashyap, L., Ferguson, A. A., Fisher, A. L. The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker. J. Vis. Exp. (47), e2331, doi:10.3791/2331 (2011).
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