JoVE Encyclopedia of Experiments
Neuroscience
0 views • 1:18 min • March 4th, 2025
Chlamydia pneumoniae are pathogenic bacteria that can infect the human brain.
To detect these bacteria, take a deparaffinized, rehydrated, bacteria-infected human brain section with exposed protein antigens and inactivated endogenous phosphatase.
Add a blocking solution to prevent non-specific antibody binding.
Incubate with primary antibodies that bind with the target bacterial antigen.
Wash with a buffer to remove unbound antibodies.
Add the blocking solution to increase the specificity of the secondary antibody.
Incubate with alkaline phosphatase-conjugated secondary antibodies, which bind to primary antibodies.
Rinse with water to remove excess antibodies.
Add a chromogenic substrate that reacts with alkaline phosphatase, forming a colored precipitate.
Rinse with water to remove excess precipitate.
Add a dye to stain nuclei.
Add mounting media to mount the section.
Under a light microscope, visualize stained bacteria in the brain tissue.