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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

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06:54 min

December 08, 2010

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06:54 min
December 08, 2010

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Summary

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Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.

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