Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Ismael C. Gomes; Mariana Acquarone; Renata de Moraes Maciel; Rafael Bierig Erlich; Stevens K. Rehen

doi:10.3791/2344

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

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06:54 min

December 8, 2010

DOI: 10.3791/2344-v

Ismael C. Gomes*¹, Mariana Acquarone*¹, Renata de Moraes Maciel*¹, Rafael Bierig Erlich¹, Stevens K. Rehen¹

¹Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro (UFRJ), Brazil

Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.

Part One, Introduction. Hi, my name is Rafael. I'm a postdoc at Professor Stevens Hans Lab at Federal University of Rio de Janeiro in Brazil.

Hi, my name is Ismail. I am a research assistant, also professor. In this video, we'll show how to prepare clear sections of Android bodies.

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