Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.
Gomes, I. C., Acquarone, M., Maciel, R. d. M., Erlich, R. B., Rehen, S. K. Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies. J. Vis. Exp. (46), e2344, doi:10.3791/2344 (2010).