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In vivo en in vitro studies van Adapter-clathrine Interactie
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In vivo en in vitro studies van Adapter-clathrine Interactie

Article DOI: 10.3791/2352-v • 17:14 min • January 26th, 2011
January 26th, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Colorado State University

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Clathrine-gemedieerde endocytose afhankelijk van de adapter eiwitten die lading selectie en clathrine vacht montage coördineren. Hier beschrijven we de procedures voor adapter-clathrine fysieke interactie en live cell imaging methoden gebruikt als een model de gist endocytische adapter eiwit Sla1p bestuderen.

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Celbiologie clathrine adapter Sla1p pull down immunoprecipitatie GFP fluorescentie microscopie
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