Journal
/
Biology
/
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

DOI:
10.3791/2362-v

•

07:53 min

•

December 10, 2010

•

, , , , , ,
1Orthopaedics Research,University of Virginia, 2Biological Sciences,University of Delaware, 3Orthopaedic Surgery,University of Virginia

Chapters

  • 00:05Title
  • 01:44Biopanning
  • 03:39Peptide Staining of Mesenchymal Cultured Cells
  • 04:42Preparation of Gel Foam Composite and In Vivo Implantation
  • 05:25Histology
  • 05:40Effects of Phage Display Peptides on Osteogenesis In Vitro and In Vivo
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these peptides on mesenchymal cell differentiation and to determine their effect on bone regeneration.

Tags

PeptidesPhage Display LibraryGene ExpressionMesenchymal CellsOsteogenesisUnicortical Bone DefectsBone FormationCollagen MatrixAlkaline Phosphatase ActivityNodule FormationRunx2OsterixBone SialoproteinOsteocalcinCollagen SpongeRepair Of Bone DefectsIn Vitro DifferentiationIn Vivo Delivery

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2362?article_type=v

Culture of myeloid dendritic cells from bone marrow precursors
check_url/2362?article_type=v

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
check_url/2362?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/2362?article_type=v

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects
check_url/2362?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/2362?article_type=v

In vitro Reconstitution of the Active T. castaneum Telomerase
check_url/2362?article_type=v

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
check_url/2362?article_type=v

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
check_url/2362?article_type=v

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
check_url/2362?article_type=v

Measurement of Metabolic Rate in Drosophila using Respirometry

Read Article