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JoVE Journal
Neuroscience

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Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
 

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Article DOI: 10.3791/2373
January 24th, 2011

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Summary January 24th, 2011

This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

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