Preparation of a Microfluidic Plate with Bacterial Cells

Take a microfluidic plate containing pairs of input and output wells connected by microfluidic channels, which include serpentine channels and an experimental channel.

To prime the channels, add media to the output well.

Place the plate into a plate stage and seal it with a sterilized cover.

Activate the media flow from the output well to the input well to flush out any trapped air pockets within the channels.

Remove the plate. Discard the residual media from the output well and add a bacterial culture.

To the input well, add fresh media to prevent air backflow. Place the plate back into the stage.

While observing under a microscope, resume the flow to draw cells into the experimental channel.

Adjust the flow rate to avoid cell entry into the serpentine channels to prevent clogging.

Stop the flow and incubate to facilitate bacterial attachment.

Remove the plate and discard the residual bacterial culture and media.

The bacteria within the microfluidic plate are now ready for further experiments.

For priming, first remove the 48-well microfluidic plate from the packaging, without touching the glass surface at the bottom of the plate, and clean the glass slide at the plate bottom with a lens tis