Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

The overall goal of this procedure is to demonstrate the fabrication and operation of a microfluidic device that recreates tumor microenvironments in vitro and can be used for testing chemotherapeutic drugs. This is the microfluidic device. This device can be useful in the following areas, easy testing for chemotherapeutic drugs in vitro.

It provides consistent and accurate results for testing three dimensional tumor tissue, and this device provides a very accurate representation of in vivo tumor. Microenvironments tumors exist in 3D microenvironments and the ability to test in one is significant. The first step of the procedure is to assemble the device, which involves fabrication of microfluidic chip using soft lithography, punching holes at the inlets and outlets, bonding to a glass slide and connecting tubing for flow inlet and outlet.