Pdms Fabrication
Pdms Fabrication - Related Videos
14:48
PDMS Device Fabrication and Surface Modification
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Cited by 5 •
2007
11:52
Microbubble Fabrication of Concave-porosity PDMS Beads
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Cited by 2 •
2015
Procedures used to generate microstructured concave-porosity polydimethylsiloxane beads are presented. Effects of electrolyte concentration and identity within the aqueous phase are particularly emphasized.
04:45
Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
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Cited by 19 •
2007
In this video we demonstrate how to use the neuron microfluidic device without plasma bonding.
09:41
Stretching Micropatterned Cells on a PDMS Membrane
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Cited by 6 •
2014
This manuscript presents a technique to apply or release forces on adherent cells or tissues using unidirectional stretching.
08:58
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
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Cited by 8 •
2015
Microfluidic double emulsions generation typically involves devices with patterned wettability or custom-fabricated glass components. Here we describe the fabrication and testing of an all polydimethylsiloxane (PDMS) double emulsion generator that does not require surface treatment or complicated fabrication processes, and is capable of producing double emulsions down to 14 µm.
FAQs
The curing agent is mixed with the liquid PDMS prepolymer before the material is shaped and heated. Thermal curing transforms this mixture into a hardened elastomer that retains the geometry of the patterned mold. This step is essential for producing stable structures such as channels and culture-device features rather than leaving a flowable liquid.
Degassing removes bubbles from the mixed PDMS before molding. Eliminating trapped air helps preserve the intended patterned structure and supports the optical clarity needed for biological observation. It is particularly relevant when fabrication produces microfluidic channels or cell culture devices, where bubbles could interfere with the defined structure used to study cells or fluid transport.
Several material properties make PDMS useful in biological research. Its transparency supports optical observation, while flexibility enables soft structures and biomimetic models. Gas permeability and compatibility with soft lithography further support controlled biological platforms. Together, these characteristics allow investigators to examine cell behavior, fluid movement, and tissue-level processes within engineered structures.
The mold provides the geometric pattern that the liquid prepolymer occupies before thermal curing. After hardening, the PDMS can be released from that mold, preserving the formed features. Researchers can therefore use patterned molds to create defined microfluidic channels and related biological devices, with the mold serving as the template for the structure under study.
A typical workflow mixes the PDMS prepolymer with curing agent, removes bubbles by degassing, pours the mixture over a patterned mold, and thermally cures it. The hardened material is then released and may be bonded to glass or another PDMS layer. This sequence converts a liquid formulation into an assembled platform for biological experiments.
PDMS fabrication supports microfluidic channels, cell culture devices, organ-on-chip platforms, and biomimetic models. These formats give researchers controlled settings for studying cell behavior, fluid transport, and tissue-level processes. Its value in biology comes from combining structured device production with transparency, elasticity, gas permeability, and compatibility with soft lithography.