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Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
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Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

Article DOI: 10.3791/2431 • 11:11 min • February 28th, 2011
February 28th, 2011 •
1, 1,2, 2, 1,2
1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, 2Faculty of Kinesiology, University of Calgary

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Summary
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Saponin-permeabilized fiber preparation in conjunction with respirometric oxidative phosphorylation analysis provides integrative assessment of mitochondrial function. Mitochondrial respiration in physiological and pathological states can reflect various regulatory influences including mitochondrial interactions, morphology and biochemistry.

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Respirometric Oxidative Phosphorylation Saponin-permeabilized Cardiac Fibers Mitochondrial Function Energy Metabolism Oxidative Stress Apoptosis Aging Mitochondrial Encephalomyopathies Drug Toxicity Clark Electrode Substrate-inhibitor-uncoupler Titration Protocols Electron Transport Chain Sarcolemma Muscle Fibers Saponin-skinned Cardiac Fibers Oxygen Consumption Measurements
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