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Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs
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Chapters
- 00:05Title
- 03:21Manufacturing: Wet Milling nano-ART by NETZSCH MicroCer Methods
- 06:44Manufacturing: Homogenizing nanoART by Avestin EmulsiFlex C5
- 07:52Manufacturing: Sonication by Cole Parmer Ultrasonic Processor
- 09:58NanoART Visualization and Morphology
- 10:56Biology of nanaART
- 15:37Representative Results
- 17:21Conclusion
Nanoparticles of indinavir, ritonavir, efavirenz and atazanavir were manufactured using wet milling, homogenization and ultrasonication. These nanoformulations, collectively termed nanoformulated antiretroviral therapy (nanoART), assessed macrophage-based drug delivery. Monocyte-derived macrophage nanoART uptake, retention and sustained release were determined. These preliminary studies suggest the potential of nanoART for clinical use.
Tags
Methods DevelopmentBlood BorneMacrophageNanoformulated Antiretroviral DrugsPharmacodynamicsBioavailabilityDrug ToxicitiesNanomedicineART RegimensComplianceDrug PharmacokineticsManufacturing NanoARTSurfactant Lipid CoatingWet MillingHigh-pressure HomogenizationPolymer SuspensionUltrasonicationPhysical PropertiesParticle SizeChargeShapeBiologic PropertiesCell UptakeRetentionCytotoxicityAntiretroviral EfficacyHuman Monocyte-derived Macrophages (MDM)Peripheral Blood Monocytes IsolationLeukopacks Purification
