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Chapters
- 00:05Title
- 01:05Replication of the Metagenomic Library Plates
- 03:39Measuring the Growth of E. coli Clones.
- 06:35Addition of the Assay Mix to Each Plate
- 07:47Reading Absorbance of Assayed Clones
- 09:17Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
- 09:56Conclusion
This protocol describes a high throughput screen for cellulolytic activity from a metagenomic library expressed in Escherichia coli. The screen is solution based and highly automated, and uses one-pot chemistry in 384 well microplates with the final readout as an absorbance measurement.
Tags
High Throughput ScreenBiomining Cellulase ActivityMetagenomic LibrariesCelluloseIndustrial ApplicationsBiofuel ProductionChemical MethodsEnzymatic MethodsCellulasesBacterial IsolatesFungal IsolatesLaboratory CultivationEnvironmental Genomic ScreeningMetagenomic Screening ApproachesSoilsBuffalo RumenTermite Hind-gutCarboxymethylcellulose (CMC) Agar PlatesCongo Red DyeMethod Of Teather And WoodSignal To Noise RatioChromogenic Dinitrophenol (DNP)-cellobioside SubstratePCC1 C
