JoVE Encyclopedia of Experiments
Microbiology
0 views • 2:27 min • September 30th, 2026
Begin with cultured kidney epithelial cells maintained in a serum-containing medium.
Replace this medium with a serum-free medium containing the genetically engineered viruses carrying immunomodulatory genes for therapeutic use.
Incubate the cells to allow the virus to attach to the cell surface receptors.
Remove the supernatant containing residual unattached virus.
Add fresh serum-containing medium for cell survival, and incubate.
During incubation, the virus enters through receptor-mediated endocytosis, releases its genome, and this genome produces the viral RNAs.
The viral genome replicates, while viral RNA produces new viral proteins.
The viral proteins assemble into new viral particles and are released into the surrounding medium.
These progeny viruses enter the neighboring cells, causing infection and forming clusters of infected cells.
Gently scrape off the cell monolayer to harvest cells producing therapeutic viral progeny.
Collect the suspension in a tube and store at a low temperature for further analysis.
For analysis of measles virus replication kinetics one day prior to infection seed 100,000 Vero cells in 1 milliliter of DMEM with 10 percent FBS per well on 12 well plates with a
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