JoVE Encyclopedia of Experiments
Microbiology
0 views • 3:01 min • September 30th, 2026
Begin with a test well containing genetically modified tumor cells expressing a target antigen, and a control well with tumor cells lacking the antigen.
Take a bispecific T-cell engager (BTE) encoded by a viral transgene, with two binding sites, one for the target antigen and another for a T cell-specific receptor.
Add the BTE and T cells to the wells and incubate.
In the test sample, the BTE crosslinks the tumor and T cell, activating the T cell to release cytolytic granules.
This lyses the tumor cell, releasing an intracellular enzyme.
In the control, the BTE binds only to the T cell, avoiding tumor cell lysis.
Centrifuge and collect the supernatant.
Add a substrate and incubate.
The enzyme catalyzes the substrate into a red-colored product.
Add a stop solution and measure the color intensity.
Higher intensity in the test sample confirms the target-specific immunomodulatory activity of the BTE.
To begin evaluation of BTE induced cell mediated cytotoxicity by LDH release, first isolate secreted transgene products expressed by virus infected target cells and isolate immune effector cells as described in the manuscript. Then seed target cells in a U-bottom 96 well plate and include samples as
View the full transcript and gain access to thousands of scientific videos