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RNAi的干扰双链RNA注射到果蝇胚胎
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JoVE Journal Biology
RNAi Interference by dsRNA Injection into Drosophila Embryos
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RNAi的干扰双链RNA注射到果蝇胚胎

DOI:
10.3791/2477-v

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08:30 min

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April 11, 2011

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, , ,
1Department of Biological Sciences,Oakland University

Chapters

  • 00:05Title
  • 02:01Embryo Collection & Lining Up
  • 03:35Needle Preparation
  • 04:40dsRNA Injection into Embryos and Phenotypic Analysis
  • 06:36Representative RNAi Results
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

RNA干扰已被证明非常有效的分析基因功能<em>果蝇</em>气管发展。说明了一个详细的协议，由江实验室用于注入飞胚胎击倒基因表达的dsRNA。这种技术的组织和器官的发育需要筛选基因的潜力<em>果蝇</em>。

Tags

RNAi InterferenceDsRNA InjectionDrosophila EmbryosGenetic ScreeningGenetic ManipulationReverse Genetic ApproachRNAiGene FunctionsOrgan DevelopmentTubular NetworksTracheal FormationMorphogenesisGene ExpressionKnockdownDysfusion Gene

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