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JoVE Journal
Biology

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Loading Drosophila Nerve Terminals with Calcium Indicators
 

Loading Drosophila Nerve Terminals with Calcium Indicators

Article DOI: 10.3791/250 15:16 min
July 30th, 2007

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Summary July 30th, 2007

Calcium is a ubiquitous messenger in the nervous system, essential for triggering neurotransmitter release and changes in synaptic strength. Here we demonstrate a technique for loading Ca2+-indicators into Drosophila nerve terminals. We also demonstrate fabrication of the required apparatus and emphasize points critical for the technique's success.

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