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影像生活细胞的形状变化果蝇胚胎
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Cell Shape Change in Living Drosophila Embryos
DOI:

11:20 min

March 30, 2011

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Chapters

  • 00:05Title
  • 00:45Introduction
  • 02:11Making the Embryo Collection Cups
  • 02:31Adding GFP Flies to Embryo Collection Cups
  • 04:43Preparing a Mounting Chamber and Mounting Embryos
  • 07:15Imaging Embryos
  • 07:55Alternative Method: Mounting Embryos with Embryo -glue”
  • 09:30Results: Time-lapse Imaging of Cellularization
  • 10:34Conclusion

Summary

Automatic Translation

果蝇,果蝇,早期发展的特点是由许多细胞形状的变化,以及成像方法适合。本文将描述活果蝇胚胎的共焦成像所需的基本工具和方法,将集中在细胞形态变化称为cellularization。

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