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热休克法转化到大肠杆菌质粒DNA
JoVE Journal
Biology
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JoVE Journal
Biology
Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
Please note that all translations are automatically generated.
Click here for the English version.
热休克法转化到大肠杆菌质粒DNA
DOI:
10.3791/253-v
•
07:46 min
•
August 01, 2007
•
Alexandrine Froger
,
James E. Hall
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:04
Introduction
00:38
Preparing for Heat Transformation
01:14
Introducing plasmid DNA into bacteria by heat shock
03:05
Plating transformed bacteria on LB agar plate
05:59
Assessing the transformation efficiency
06:42
Conclusion
Tags
Transformation
Plasmid DNA
E. Coli
Heat Shock Method
Molecular Biology
Foreign Plasmid
Ligation Product
Chemically Competent Bacteria
Genlantis
Incubation
Ice
DNA
Heat Shock
SOC Media
Transformed Cells
Agitation
Colonies
Transformation Efficiency
Competent Bacteria
Turbocells
3-minute Transformation Protocol
Article
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