Dünndarmtransplantation In Mice
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Zitiert von 2
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36:30 Min.
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20. August 2007
20. August 2007
•Die Maus Dünndarmtransplantation-Modell ist als ein wichtiges Instrument zur mechanismes der Immunabwehr und Bildschirm neue immunsuppressive Medikamente Studie erkannt worden. Allerdings ist dieses Modell beschränkt zu verwenden, da die Techniken handelt sich um eine technisch äußerst Herausforderung. Wir führen nun die modifizierte Technik.
Chapters in this video
0:03
Title
0:16
Introduction
1:18
Harvesting Small Bowel from Donor Mouse
1:20
1. performing Tail Pinch to Ensure Mouse is Anesthetized
2:46
2. clearing away tissue to allow ligation of small bowel
4:21
3. cleaning out small bowel contents before ligating ileum
4:50
4. ligating ileum
5:20
5. exposing and ligating mesenteric vessels
6:18
6. exposing mesenteric artery which will be cut when small bowel is
6:42
7. exposing portal vein
7:28
8. ligating branch of portal vein
7:58
9. exposing other side of mesenteric artery
8:09
10. locating inferior vena cava
8:15
11. injecting heparin into the inferior vena cava
8:36
12. cutting mesenteric artery
8:54
13.clearing away tissue from mesentaric artery
9:23
14. cutting jejunal end of ligated small bowel
9:37
15. cutting away small bowel mesenteric tissue
10:27
16. cutting away mesenteric tissue until the portal vein is reached
10:56
17. cutting ileal end of ligated small bowel
11:19
18. cutting away mesenteric tissue
11:34
19. cutting portal vein
11:57
20. harvesting donor small bowel
12:09
Preparing Recipient Mouse for Small Bowel Transplantation
13:20
1. exposing inferior vena cava and aorta
13:29
2. clearing away tissue in order to apply ligatures
14:35
3. applying ligatures to inferior vena cava and inferior aorta
15:03
Connecting Donor Small Bowel Circulation to Recipient Circulation
15:15
1. placing donor small bowel in recipient mouse
15:56
puncturing inferior vena cava for veinotomy
16:17
3. performing veinotomy on inferior vena cava
16:31
4. repeat procedure for aorta
17:00
5. washing away blood from vessels with saline
17:15
6. suturing donor mesenteric artery to recipient aorta
19:59
7. suturing donor portal vein to recipient inferior vena cava
22:39
8. final suture for anastamosis of donor portal vein to recipient i
23:01
9. flipping donor small bowel in order to finish suture of mesenter
23:35
10. suturing donor mesenteric artery to other side of aorta
24:53
11. final suture for anastamosis of donor mesenteric artery to reci
25:56
12. releasing ligatures to open circulation to donor small bowel
26:47
Connecting Donor Small Bowel to Recipient Small Bowel
27:20
1. clamping ileal end of donor small bowel
27:39
2. cutting recipient jejunum
27:50
3. cutting ileal end of donor small bowel
28:04
4. suturing one side of donor ileum to recipient jejunum
29:10
5. flipping small bowel clamps in order to suture other side of ana
30:20
6. removing clamps
31:14
7. creating stoma for jejunal end of donor small bowel
32:02
8. suturing jejunal end of donor small bowel to stoma
32:40
9. cutting ligature to jejunal end of donor small bowel
33:14
Sewing up Abdominal Cavity
35:32
Conclusion
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