Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

Suresh Garudadri; Benjamin Gallarda; Samuel Pfaff; William Alaynick

doi:10.3791/2580

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

JoVE Journal
Neuroscience
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JoVE Journal Neuroscience

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

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08:47 min

February 20, 2011

DOI: 10.3791/2580-v

Suresh Garudadri¹, Benjamin Gallarda¹, Samuel Pfaff¹, William Alaynick¹

¹Howard Hughes Medical Institute and Gene Expression Laboratory,The Salk Institute for Biological Studies

A demonstration of the fabrication and use of an extracellular suction electrode used to measure electrophysiological recordings of neonatal rodent spinal cords in vitro

Hi.My name is Resh Gary Dory, and I'm a research assistant in the faf Lab of the Salk Institute for Biological Studies. Today I'll be showing you how to fabricate extracellular suction electrodes using two methods. These electrodes can be used to take electrophysiological recordings of my spinal cords in vitro.

Use an alcohol lamp as a heat source to melt the PE 90 plastic tubing into the desired width. Hold the tubing over the lit flame and remove as it begins to melt, gently pull the ends apart, being careful not to break the middle. This process is shown again.

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