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Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

DOI:
10.3791/2595-v

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04:37 min

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February 24, 2011

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, , , , , , ,
1Department of Kinesiology, Recreation, and Sport,Western Kentucky University, 2Department of Health and Human Performance,University of Houston

Chapters

  • 00:01Title
  • 00:29Finger-Stick Blood Sampling
  • 01:20Cell Phenotyping and Apoptosis Staining
  • 03:53Representative Results
  • 04:05Conclusion

Summary

Automatic Translation

Automatic Translation

Exercise is capable of inducing apoptosis in immune cells. There are various measurement limitations, particularly relating to the amount of time required to isolate and treat a blood sample prior to the assessment. Demonstrated is a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte apoptosis.

Tags

Finger-stick Blood SamplingExercise-induced Lymphocyte ApoptosisImmune CellsMeasurement LimitationsTime RequiredIsolation And Treatment Of Blood SampleAssessment Of Cell DeathEffect Of Exercise On The Immune SystemRapid And Minimally Invasive ProcedureAnalysis Of Exercise-induced Lymphocyte ApoptosisAntibody PanelIncubation PeriodRed Blood Cells LysisVolume Of Blood RequiredDiscomfort To Subjects

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