/
/
Gravação de células inteiras a partir de uma preparação Slice organotípicas de Neocortex
A subscription to JoVE is required to view this content. Sign in or start your free trial.
14,084 Views
•
10:29 min
•
June 03, 2011
DOI:
Summary
Automatically generated
Este é um protocolo para preparar e manter uma preparação fatia neocortical na cultura organotípicas com a finalidade de fazer gravações elétrica de neurônios piramidais.
Explore More Videos
Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons
Read Article
Cite this Article
Foehring, R. C., Guan, D., Toleman, T., Cantrell, A. R. Whole Cell Recording from an Organotypic Slice Preparation of Neocortex. J. Vis. Exp. (52), e2600, doi:10.3791/2600 (2011).
Copy