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Immunology and Infection
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竞争的原点实验研究肠嗜T细胞迁移
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Competitive Homing Assays to Study Gut-tropic T Cell Migration
Please note that all translations are automatically generated.
Click here for the English version.
竞争的原点实验研究肠嗜T细胞迁移
DOI:
10.3791/2619-v
•
10:25 min
•
March 01, 2011
•
Eduardo J. Villablanca
,
J. Rodrigo Mora
1
Gastrointestinal Unit
,
Massachusetts General Hospital, Harvard Medical School
Chapters
00:05
Title
01:13
Introduction
01:59
Ex vivo Generation of Gut-Homing and Control T cells
04:25
Analysis of Gut-Homing Receptors on Activated T cells
05:21
T cell Labeling with CFSE and CMTMR Cell Trackers
07:42
Analysis of T cell Homing
08:58
RA-induced Expression of a4B7 and CCR9 Direct T Cell Migration to the Gut
09:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
竞争归巢实验允许直接评估一个鼠标的两个不同的细胞群的洄游特性。在这里,我们说明了此过程中,通过对比前体内产生的肠道热带与非肠嗜T细胞迁移。
Tags
Competitive Homing Assays
Gut-tropic T Cell Migration
Lymphocyte Adhesion
Adhesion Molecules
Homing Receptors
Addressins
Endothelial Cells
Intravital Microscopy (IVM)
Homing Experiments
Migration Analysis
Cell Subsets
Mouse Model
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