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全安装胚胎肢体皮肤血管的免疫组织化学分析:一个模型系统,研究在胚胎血管分支形态发生
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Biology
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JoVE Journal
Biology
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Please note that all translations are automatically generated.
Click here for the English version.
全安装胚胎肢体皮肤血管的免疫组织化学分析:一个模型系统,研究在胚胎血管分支形态发生
DOI:
10.3791/2620-v
•
09:53 min
•
May 20, 2011
•
Wenling Li
,
Yoh-suke Mukouyama
1
Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center
,
National Heart, Lung, and Blood Institute, National Institutes of Health
Chapters
00:05
Title
01:42
Collecting Embryonic Limb Skins
03:53
Whole-mount Immunohistochemical Staining of Limb Skins
07:14
Mounting the Limb Skins on a Slide
08:24
Results of Whole-mount Confocal Immunofluorescence Microscopy
09:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍整个安装的免疫组织化学和激光扫描共聚焦显微镜分析复杂的小鼠胚胎肢体皮肤血管网的形成多个标签。
Tags
Whole-mount Immunohistochemical Analysis
Embryonic Limb Skin Vasculature
Vascular Branching Morphogenesis
Vascular Development
Limb Skin Vasculature Model
Whole-mount Confocal Microscopy
Fluorescent Markers
Genetic Studies
Molecular Mechanisms
Peripheral Nerves
Spatial Template
Differentiation And Patterning Of Arteries
Staining Intact Blood Vessels
Vascular Specific Antibodies
Fluorescent Secondary Antibodies
Vascularized Embryonic Organs
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