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Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

DOI:
10.3791/2659-v

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08:50 min

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June 02, 2011

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, , , ,
1Institute for Molecular and Clinical Immunology,Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation,Helmholtz Center for Infection Research

Chapters

  • 00:05Title
  • 01:29Spore Preparation
  • 02:54Infection
  • 04:05Lung Preparation
  • 06:332-Photon Laser Microscopic Visualization of Neutrophils and NETs in Control and Aspergillus fumigatis-infected Lung Tissue
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

Tags

PhagocytosisNET-formationNeutrophilsInfected Lungs2-photon MicroscopyGas ExchangeDefense StrategiesHumoral Immune MechanismsCellular Immune MechanismsRecruitment Of NeutrophilsCytotoxic ChemicalsExtracellular DNA-NETsBacteriaFungiLung InfectionViable Lung SlicesIntratracheal InfectionAspergillus FumigatusMulticolor Time-lapse Microscopy

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