Lys Egfp Mice
Lys Egfp Mice - Related Videos
11:43
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
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Cited by 37 •
2014
This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.
10:03
Development of Leishmania Species Strains with Constitutive Expression of eGFP
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2023
Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
10:20
Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
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Cited by 1 •
2022
Here, non-invasive methods are described for localization of photoreceptor membrane proteins and assessment of retinal degeneration in the Drosophila compound eye using eGFP fluorescence.
10:25
Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
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Cited by 11 •
2012
This article describes an optimized sequence of events for multimodal imaging of cellular grafts in rodent brain using: (i) in vivo bioluminescence and magnetic resonance imaging, and (ii) post mortem histological analysis. Combining these imaging modalities on a single animal allows cellular graft evaluation with high resolution, sensitivity and specificity.
10:54
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression
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Cited by 9 •
2017
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using RNA-seq.
FAQs
The lysozyme M regulatory sequences direct enhanced green fluorescent protein expression in selected myeloid populations. Because these cells carry the fluorescent reporter within the animal, fluorescence microscopy can reveal where they are located and how they move through living tissues. This makes recruitment, migration, and tissue infiltration observable as dynamic processes rather than only endpoint measurements.
Fluorescence occurs predominantly in neutrophils, monocytes, and macrophage-lineage cells. This labeling connects the observed signal to major innate immune-cell populations involved in inflammation and host defense. In an experiment, their distribution and movement can therefore provide context for how myeloid cells reach affected tissue and interact with pathogens or infected cells.
Lys-EGFP mice allow myeloid-cell localization and movement to be monitored directly in living tissues by fluorescence microscopy. Repeated cell isolation is not required for every observation, which helps preserve information about spatial position and cellular dynamics. The approach is consequently useful when tissue infiltration or cell-to-cell interaction is central to the research question.
A typical study uses the reporter animals as the source of fluorescently labeled myeloid cells, places the relevant living tissue under fluorescence microscopy, and records the cells’ locations or movements. Researchers can then examine recruitment, migration, infiltration, or interactions with pathogens and infected cells. The specific observation depends on the biological process being investigated.
In infection studies, fluorescence imaging can follow how labeled neutrophils, monocytes, and macrophage-lineage cells respond in tissues containing pathogens or infected cells. Researchers can assess where these populations accumulate, how they move, and whether they contact affected targets. These observations help connect innate immune-cell behavior with host defense and inflammatory responses.
Imaging can provide spatial and dynamic information about myeloid immune cells, including their localization, migration, tissue infiltration, and interactions with pathogens or infected cells. Rather than relying only on isolated-cell measurements, investigators can relate cell behavior to its tissue setting. This supports analysis of immune-cell dynamics during inflammation and infection.
The model links fluorescent visualization with the activity of myeloid cells that participate in innate immune responses. In immunology, this enables researchers to examine how these cells are recruited and distributed during inflammation, while infection studies can relate their behavior to pathogens or infected cells. The resulting tissue-level view supports investigation of host defense mechanisms.