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JoVE Journal
Neuroscience

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High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
 

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Article DOI: 10.3791/2661
February 1st, 2011

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Summary February 1st, 2011

The EpiMark 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a spe cific locus. The kit distinguishes 5-mC from 5-hmC by the addition of glucose to the hydroxyl group of 5-hmC via an enzymatic reaction utilizing β-glucosyltransferase (T4-BGT). When 5-hmC occurs In the context of CCGG, this modification converts a cleavable MspI site to a non-cleavable site.

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