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Chapters
- 00:05Title
- 01:02Preparation for Dissecting and Immunofluorescent Staining
- 01:25Dissection of Third-instar Larvae
- 03:05Removal of Excess Tissue for Immunofluorescence
- 03:41Mounting of Immunostained Larval Pelts
- 05:12Larval Mounting and Immunostaining Results
- 05:50Conclusion
A junção neuromuscular (MNJ) de<em> Drosophila melanogaster</em> É um sistema modelo importante para o estudo da função sináptica normal, assim como perturbações de função sináptica encontrada em certas doenças neurológicas. Nós apresentamos um protocolo para a dissecção da<em> Drosophila</em> Larval sistema motor e imunomarcação de proteínas zona ativa dentro do MNJ.
Tags
DissectionImagingActive ZonesDrosophila Neuromuscular JunctionSynaptic StructureSynaptic FunctionGenetic ManipulationsLarval Nervous SystemNeurological DiseaseSynaptic MoleculesGlutamatergicActivity-dependent RemodelingIdentified Synaptic TerminalsMotor NeuronsBody-wall Muscle FibersEvolutionarily Conserved Synapse ComponentsMechanisms Underlying Synaptic FunctionMonoclonal AntibodyBruchpilot (Brp)CAST1/ERC Family Member
